LOCUS       pLV-[hsa-miR-220b]        9141 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7062..7922
                     /label=Amp(R)
     promoter        8123..8663
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5667..5901
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6476..6931)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5973..6103
                     /label=SV40 pA
     misc_feature    6104..6317
                     /label=SV40 ori
     misc_feature    4988..5584
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3606
                     /label=intron
     CDS             3653..4978
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3396
                     /label=hsa-mir-220b precursor
BASE COUNT     2148 a      2391 c      2496 g      2106 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc agcttgaggg 
     3121 tgcggaaaca gatgtcgtag agtgcctcgt tgtcgatgca gtaggtctca tccgtattct 
     3181 ccaccagctg gtgcacagac agcgtggcgt tgtagggctc caccaccgtg tctgacactt 
     3241 tgggcgaggg caccacgctg aaggtgttca tgatgcggtc tgggaactcc tcgcggatct 
     3301 tactgatgag cagcgtgccc attccggacc ccgtgccacc ccccagcgag tgggtcagct 
     3361 ggaagccctg aaggcagtcg cagctctcgg cctcctgcta gctcgagctt ttggagtacg 
     3421 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg 
     3481 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt 
     3541 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca 
     3601 tttcaggtgt cgtgaaaact acccctctag agtcgagcta ccggtcgcca ccatggtgag 
     3661 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 
     3721 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 
     3781 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 
     3841 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga 
     3901 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 
     3961 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt 
     4021 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa 
     4081 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa 
     4141 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa 
     4201 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa 
     4261 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga 
     4321 gggccgccac tccaccggcg gcatggacga gctgtacaag gacccaccgg tcgccaccat 
     4381 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 
     4441 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 
     4501 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 
     4561 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 
     4621 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 
     4681 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 
     4741 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 
     4801 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 
     4861 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 
     4921 cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgagc 
     4981 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
     5041 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
     5101 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
     5161 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
     5221 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
     5281 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
     5341 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
     5401 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
     5461 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
     5521 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 
     5581 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 
     5641 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     5701 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     5761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     5821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     5881 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     5941 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 
     6001 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     6061 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 
     6121 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     6181 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     6241 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 
     6301 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     6361 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     6421 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     6481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     6541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     6601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     6661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     6721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     6781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     6841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     6901 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     6961 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     7021 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     7081 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     7141 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     7201 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     7261 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     7321 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     7381 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     7441 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     7501 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     7561 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     7621 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     7681 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     7741 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     7801 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     7861 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     7921 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     7981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     8041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     8101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     8161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     8221 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     8281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     8341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     8401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     8461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     8521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     8581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     8641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     8701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     8761 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     8821 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
     8881 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     8941 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
     9001 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
     9061 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 
     9121 gggaacaaaa gctggagctg c 
//
